Leptosperin

Leptosperin is the first of what could be a number of Manuka specific chemical markers, which are used to identify Manuka honey for authenticity and labelling purposes.

Leptosperin is a naturally occurring chemical, found only in the nectar of Manuka plants (and a few very close relatives).  It was first identified (and patented) by Japanese researcher Kato in 2014*, and later that year its presence in Manuka nectar was reported by the UMFHA and Analytica Laboratories at the Apiculture Industry Conference in Whanganui, New Zealand.  Leptosperin concentration is stable over time, and therefore measuring its concentration in honey is a good way of identifying whether the honey comes from Manuka nectar, and whether it can be labelled as Manuka, or a Manuka blend, or as a multi-floral honey.

Leptosperin concentrations have the ability to highlight cases of possible adulteration of honey to look like Manuka.

At this stage, UMFHA holds the NZ license for the use of Leptosperin testing in Manuka honey, and ask us to collect a royalty for all tests carried out.  UMFHA members pay a $5 + GST per test royalty and non-members pay a $10 + GST per test royalty.  Royalties are collected by Analytica for payment in full to the UMFHA.

*Kato Y, Fujinaka R, Ishisaka A, Nitta Y, Kitamoto N, Takimoto Y. Plausible authentication of manuka honey and related products by measuring leptosperin with methyl syringate. J. Agric. Food Chem., 2014, 62 (27), pp 6400–6407

How does Analytica conduct the test

A sub sample of honey is extracted in ultra-pure water, diluted and analysed by Ultra Performance Liquid Chromatography (UPLC).

Why Use Analytica?

Analytica developed the leptosperin method on behalf of Unique Manuka Factor® Honey Association (UMFHA) and was the first lab in New Zealand offering this testing.

Leptosperin only

Test / Price / Turnaround
Leptosperin only
$40.00 + $5.00 UMFHA royalty (UMFHA members) or $10 + GST UMFHA royalty (non-members)
1-2 days
Manuka 3-in-1 (DHA+MG+HMF+NPA/UMF) + Leptosperin
$70.00 + $5.00 UMFHA royalty (UMFHA members) or $10 + GST UMFHA royalty (non-members)
1-2 Days

How to take and submit a sample

How to take and submit a sample


To have your honey tested, send approximately 50 g of honey in a plastic pottle with a screw lid. A representative sample is required. The sample should not contain wax.

Download and complete our Analysis Request Form either in PDF format or as a Word document, and send it along with your samples to:

Analytica Laboratories Ltd
Ruakura Research Centre
10 Bisley Road,
Hamilton 3240 
New Zealand

Analysis Request Form | PDF

Analysis Request Form | WORD

Indicate in the columns the samples that require just Leptosperin or Leptosperin and “3-in-1” testing (this requires ticking both the 3-in-1 column and the Leptosperin column).

Don’t forget to label each sample container with a unique name or code, matching the Sample Identification that you write on the Analysis Request Form. We will invoice you when we report your results. 

Please email the Sample Reception team at sample.receipt@analytica.co.nz if you are sending more than 40 samples to make the most of our testing prices and turnaround times.

To send samples from overseas, please see our instructions and import permits.

*For some ideas on collecting a representative honey sample, have a look at this article published in 'The New Zealand BeeKeeper'.


Analytica Laboratories

P: +64 7 974 4740
E: sales@analytica.co.nz

Ruakura Research Centre,
10 Bisley Road, Private Bag 3123,
Hamilton 3214
New Zealand


IANZ Accredited Laboratory

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